Product Information
- Product Type
- Monoclonal antibody
- Clone Number
- 3E5
- UniProt No.
- P63027
- NCBI Accession No.
- NP_055047
- Alternative names
- vesicle-associated membrane protein 2, VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2,Vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 FLJ11460,RATVAMPB, RATVAMPIR, SYB, VAMP 2, Vesicle associated membrane protein 2, Vesicle-associatedmembrane protein 2 (synaptobrevin 2)
- Additional Information
- This product was produced from tissue culture supernatant.
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Synaptobrevin 2 (1-89aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-A affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of VAMP-2 in U87MG cells. The cell was stained with ATGA0588 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human VAMP1 protein
Lane 2.: Recombinant human VAMP2 protein
Lane 3.: Recombinant human VAMP3 protein
Lane 1.: Recombinant human VAMP1 protein
Lane 2.: Recombinant human VAMP2 protein
Lane 3.: Recombinant human VAMP3 protein
The tissue lysate(10ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Brain Tissue lysate
Lane 1.: Mouse Brain Tissue lysate
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.