NKMAX

Product Information

Product Type

cDNA

Antigen Species

Human

NCBI Accession No.

NP_002101.2

Alternative names

Tyrosine-protein kinase HCK, HCK proto-oncogene, Src family tyrosine kinase, Hemopoietic cell kinase, JTK9, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck

RNA Reference Number

NM_002110.3

OMIM Number

142370

Chromosome Location

20q11-q12

Product Specification

Formulation

Lyophilized

Storage

Store the plasmid at -20C.

cDNA size

1581bp

Preparation before usage

1. Centrifuge at 7000rpm for 1 minute.
2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA.
Each tube contains approximately 10ug of lyophilized plasmid.

Vector description:

This shuttle vector contains the complete ORF. It is inseted Nde I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.

General Description

HCK encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon.

Data

1. Nucleotide Sequence:
   

   CTGGGGGGGC GCTCAAGCTG CGAGGATCCG GGCTGCCCGC GAGACGAGGA GCGGGCGCCC AGGATGGGGT GCATGAAGTC CAAGTTCCTC CAGGTCGGAG GCAATACATT CTCAAAAACT GAAACCAGCG CCAGCCCACA CTGTCCTGTG TACGTGCCGG ATCCCACATC CACCATCAAG CCGGGGCCTA ATAGCCACAA CAGCAACACA CCAGGAATCA GGGAGGCAGG CTCTGAGGAC ATCATCGTGG TTGCCCTGTA TGATTACGAG GCCATTCACC ACGAAGACCT CAGCTTCCAG AAGGGGGACC AGATGGTGGT CCTAGAGGAA TCCGGGGAGT GGTGGAAGGC TCGATCCCTG GCCACCCGGA AGGAGGGCTA CATCCCAAGC AACTATGTCG CCCGCGTTGA CTCTCTGGAG ACAGAGGAGT GGTTTTTCAA GGGCATCAGC CGGAAGGACG CAGAGCGCCA ACTGCTGGCT CCCGGCAACA TGCTGGGCTC CTTCATGATC CGGGATAGCG AGACCACTAA AGGAAGCTAC TCTTTGTCCG TGCGAGACTA CGACCCTCGG CAGGGAGATA CCGTGAAACA TTACAAGATC CGGACCCTGG ACAACGGGGG CTTCTACATA TCCCCCCGAA GCACCTTCAG CACTCTGCAG GAGCTGGTGG ACCACTACAA GAAGGGGAAC GACGGGCTCT GCCAGAAACT GTCGGTGCCC TGCATGTCTT CCAAGCCCCA GAAGCCTTGG GAGAAAGATG CCTGGGAGAT CCCTCGGGAA TCCCTCAAGC TGGAGAAGAA ACTTGGAGCT GGGCAGTTTG GGGAAGTCTG GATGGCCACC TACAACAAGC ACACCAAGGT GGCAGTGAAG ACGATGAAGC CAGGGAGCAT GTCGGTGGAG GCCTTCCTGG CAGAGGCCAA CGTGATGAAA ACTCTGCAGC ATGACAAGCT GGTCAAACTT CATGCGGTGG TCACCAAGGA GCCCATCTAC ATCATCACGG AGTTCATGGC CAAAGGAAGC TTGCTGGACT TTCTGAAAAG TGATGAGGGC AGCAAGCAGC CATTGCCAAA ACTCATTGAC TTCTCAGCCC AGATTGCAGA AGGCATGGCC TTCATCGAGC AGAGGAACTA CATCCACCGA GACCTCCGAG CTGCCAACAT CTTGGTCTCT GCATCCCTGG TGTGTAAGAT TGCTGACTTT GGCCTGGCCC GGGTCATTGA GGACAACGAG TACACGGCTC GGGAAGGGGC CAAGTTCCCC ATCAAGTGGA CAGCTCCTGA AGCCATCAAC TTTGGCTCCT TCACCATCAA GTCAGACGTC TGGTCCTTTG GTATCCTGCT GATGGAGATC GTCACCTACG GCCGGATCCC TTACCCAGGG ATGTCAAACC CTGAAGTGAT CCGAGCTCTG GAGCGTGGAT ACCGGATGCC TCGCCCAGAG AACTGCCCAG AGGAGCTCTA CAACATCATG ATGCGCTGCT GGAAAAACCG TCCGGAGGAG CGGCCGACCT TCGAATACAT CCAGAGTGTG CTGGATGACT TCTACACGGC CACAGAGAGC CAGTACCAAC AGCAGCCATG A

2. Translation Sequence:
   LGGRSSCEDP GCPRDEERAP RMGCMKSKFL QVGGNTFSKT ETSASPHCPV YVPDPTSTIK PGPNSHNSNT PGIREAGSED IIVVALYDYE AIHHEDLSFQ KGDQMVVLEE SGEWWKARSL ATRKEGYIPS NYVARVDSLE TEEWFFKGIS RKDAERQLLA PGNMLGSFMI RDSETTKGSY SLSVRDYDPR QGDTVKHYKI RTLDNGGFYI SPRSTFSTLQ ELVDHYKKGN DGLCQKLSVP CMSSKPQKPW EKDAWEIPRE SLKLEKKLGA GQFGEVWMAT YNKHTKVAVK TMKPGSMSVE AFLAEANVMK TLQHDKLVKL HAVVTKEPIY IITEFMAKGS LLDFLKSDEG SKQPLPKLID FSAQIAEGMA FIEQRNYIHR DLRAANILVS ASLVCKIADF GLARVIEDNE YTAREGAKFP IKWTAPEAIN FGSFTIKSDV WSFGILLMEI VTYGRIPYPG MSNPEVIRAL ERGYRMPRPE NCPEELYNIM MRCWKNRPEE RPTFEYIQSV LDDFYTATES QYQQQP