NKMAX

Product Information

Product Type

cDNA

Antigen Species

Human

NCBI Accession No.

NP_005980.1

Alternative names

Aldo-keto reductase family 1 member D1, SRD5B1, Delta 4-3-ketosteroid-5-beta-reductase

RNA Reference Number

NM_005989.3

OMIM Number

604741

Chromosome Location

7q32-q33

Product Specification

Formulation

Lyophilized

Storage

Store the plasmid at -20C.

cDNA size

981bp

Preparation before usage

1. Centrifuge at 7000rpm for 1 minute.
2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA.
Each tube contains approximately 10ug of lyophilized plasmid.

Vector description:

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.

General Description

Aldo-keto reductase family 1, member D1, also known as AKR1D1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones which carry a delta (4) -3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction.

Data

1. Nucleotide Sequence:
   

   ATGGATCTCA GTGCTGCAAG TCACCGCATA CCTCTAAGTG ATGGAAACAG CATTCCCATC ATCGGACTTG GTACCTACTC AGAACCTAAA TCGACCCCTA AGGGAGCCTG TGCAACATCG GTGAAGGTTG CTATTGACAC AGGGTACCGA CATATTGATG GGGCCTACAT CTACCAAAAT GAACACGAAG TTGGGGAGGC CATCAGGGAG AAGATAGCAG AAGGAAAGGT GCGGAGGGAA GATATCTTCT ACTGTGGAAA GCTATGGGCT ACAAATCATG TCCCAGAGAT GGTCCGCCCA ACCCTGGAGA GGACACTCAG GGTCCTCCAG CTAGATTATG TGGATCTTTA CATCATTGAA GTACCCATGG CCTTTAAGCC AGGAGATGAA ATATACCCTA GAGATGAGAA TGGCAAATGG TTATATCACA AGTCAAATCT GTGTGCCACT TGGGAGGCGA TGGAAGCTTG CAAAGACGCT GGCTTGGTGA AATCCCTGGG AGTGTCCAAT TTTAACCGCA GGCAGCTGGA GCTCATCCTG AACAAGCCAG GACTCAAACA CAAGCCAGTC AGCAACCAGG TTGAGTGCCA TCCGTATTTC ACCCAGCCAA AACTCTTGAA ATTTTGCCAA CAACATGACA TTGTCATTAC TGCATATAGC CCTTTGGGGA CCAGTAGGAA TCCAATCTGG GTGAATGTTT CTTCTCCACC TTTGTTAAAG GATGCACTTC TAAACTCATT GGGGAAAAGG TACAATAAGA CAGCAGCTCA AATTGTTTTG CGTTTCAACA TCCAGCGAGG GGTGGTTGTC ATTCCTAAAA GCTTTAATCT TGAAAGGATC AAAGAAAATT TTCAGATCTT TGACTTTTCT CTCACTGAAG AAGAAATGAA GGACATTGAA GCCTTGAATA AAAATGTCCG CTTTGTAGAA TTGCTCATGT GGCGCGATCA TCCTGAATAC CCATTTCATG ATGAATACTG A

2. Translation Sequence:
   MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY