NKMAX

Product Information

Product Type

cDNA

Antigen Species

Human

NCBI Accession No.

NP_003339.1

Alternative names

Ubiquitin-conjugating enzyme E2N, UBC13, UbcH-ben

RNA Reference Number

NM_003348.3

OMIM Number

603679

Chromosome Location

12q22

Product Specification

Formulation

Lyophilized

Storage

Store the plasmid at -20C.

cDNA size

459bp

Preparation before usage

1. Centrifuge at 7000rpm for 1 minute.
2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA.
Each tube contains approximately 10ug of lyophilized plasmid.

Vector description:

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.

General Description

uBE2N, also known as uBC13, is a member of the E2 ubiquitin-conjugating enzyme family. It catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process that does not lead to proteasomal degradation. It mediates the transcription of several target genes and is thought to play a role in cell cycle progression; cellular differentiation and DNA repair mechanisms that ensure cell survival after DNA damage.

Data

1. Nucleotide Sequence:
   

   ATGGCCGGGC TGCCCCGCAG GATCATCAAG GAAACCCAGC GTTTGCTGGC AGAACCAGTT CCTGGCATCA AAGCCGAACC AGATGAGAGC AACGCCCGTT ATTTTCATGT GGTCATTGCT GGCCCTCAGG ATTCCCCCTT TGAGGGAGGG ACTTTTAAAC TTGAACTATT CCTTCCAGAA GAATACCCAA TGGCAGCCCC TAAAGTACGT TTCATGACCA AAATTTATCA TCCTAATGTA GACAAGTTGG GAAGAATATG TTTAGATATT TTGAAAGATA AGTGGTCCCC AGCACTGCAG ATCCGCACAG TTCTGCTATC GATCCAGGCC TTGTTAAGTG CTCCCAATCC AGATGATCCA TTAGCAAATG ATGTAGCGGA GCAGTGGAAG ACCAACGAAG CCCAAGCCAT AGAAACAGCT AGAGCATGGA CTAGGCTATA TGCCATGAAT AATATTTAA

2. Translation Sequence:
   MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI