NKMAX

Product Information

Product Type

cDNA

Antigen Species

Human

NCBI Accession No.

NP_002693.3

Alternative names

BRN5, MPOU, TCFB1

RNA Reference Number

NM_002702.3

OMIM Number

Chromosome Location

12q13.13

Product Specification

Formulation

Lyophilized

Storage

Store the plasmid at -20C.

cDNA size

906bp

Preparation before usage

1. Centrifuge at 7000rpm for 1 minute.
2. Carefully open the vial and add 100ul of sterile water to dissolve the DNA.
Each tube contains approximately 10ug of lyophilized plasmid.

Vector description:

This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.

General Description

POU6F1 belongs to the POu transcription factor family. This protein is transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3'). In the embryo, it is expressed exclusively in the developing brain, whereas in the adult its expression is restricted to brain, heart, skeletal muscle and lung. In the brain, the highest expression levels are found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.

Data

1. Nucleotide Sequence:
   

   ATGCCAGGGATCAGCAGTCAGATCCTCACCAATGCTCAGGGACAGGTTATTGGAACCCTTCCATGGGTAGTGAACTCAGCTAGTGTGGCGGCCCCAGCACCAGCCCAAAGCCTGCAGGTCCAGGCCGTGACCCCCCAGCTGTTGTTGAACGCCCAGGGCCAGGTGATTGCGACCCTGGCTAGCAGCCCCCTGCCTCCACCTGTGGCTGTCCGGAAGCCAAGCACACCTGAGTCCCCTGCTAAGAGTGAGGTGCAGCCCATCCAGCCCACACCAACCGTGCCCCAGCCTGCTGTGGTCATTGCCAGCCCAGCTCCAGCCGCCAAGCCATCTGCCTCTGCTCCTATCCCAATTACCTGCTCAGAGACCCCCACCGTCAGCCAGTTGGTGTCCAAGCCACATACTCCAAGTCTGGATGAGGATGGGATCAACTTAGAAGAGATCCGGGAGTTTGCCAAGAACTTTAAGATCCGGCGGCTCTCGCTGGGCCTTACACAGACCCAGGTGGGTCAGGCTCTGACTGCAACGGAAGGTCCAGCCTACAGCCAGTCAGCCATCTGCCGGTTCGAGAAGCTAGACATCACACCCAAGAGTGCCCAGAAGCTAAAGCCGGTGCTGGAAAAGTGGCTAAACGAAGCTGAACTGCGGAACCAGGAAGGCCAGCAGAACCTGATGGAGTTTGTGGGAGGCGAGCCCTCCAAGAAACGCAAACGCCGCACCTCCTTCACCCCCCAGGCCATAGAGGCTCTCAATGCCTATTTTGAGAAGAACCCACTGCCCACAGGCCAGGAGATCACTGAAATTGCTAAGGAGCTCAACTACGACCGTGAGGTAGTGCGGGTCTGGTTCTGCAATCGGCGCCAGACGCTCAAGAACACCAGCAAGCTGAACGTCTTTCAGATCCCTTAG

2. Translation Sequence:
   MPGISSQILT NAQGQVIGTL PWVVNSASVA APAPAQSLQV QAVTPQLLLN AQGQVIATLASSPLPPPVAV RKPSTPESPA KSEVQPIQPT PTVPQPAVVI ASPAPAAKPS ASAPIPITCSETPTVSQLVS KPHTPSLDED GINLEEIREF AKNFKIRRLS LGLTQTQVGQ ALTATEGPAYSQSAICRFEK LDITPKSAQK LKPVLEKWLN EAELRNQEGQ QNLMEFVGGE PSKKRKRRTSFTPQAIEALN AYFEKNPLPT GQEITEIAKE LNYDREVVRV WFCNRRQTLK NTSKLNVFQIP