Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-382aa
- UniProt No.
- P35813
- NCBI Accession No.
- NP_066283
- Alternative names
- Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2CA, PP2C alpha, PDPC 1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43
Product Specification
- Molecular Weight
- 46.6 kDa (418aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 10mM Tris-HCl buffer (pH 7.5) containing 50mM NaCl, 2mM DTT, 1mM MnCl2, 20%glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 1,400unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.