Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 18-144aa
- UniProt No.
- P04141
- NCBI Accession No.
- NP_000749
- Alternative names
- Granulocyte-macrophage colony stimulating factor, CSF2, GMCSF, GM-CSF, GM-CSF, Colony-stimulating factor, CSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulating factor, Granulocyte-macrophage colony stimulating factor Burst Promoting Activity, CMCSF, Colony stimulating factor, Colony Stimulating Factor 2, CSF 2, CSF Alpha, Eosinophil Colony Stimulating Factor,Granulocyte Macrophage Colony Stimulating Factor, MGC131935, MGC138897, Pluripoietin Alpha,
Product Specification
- Molecular Weight
- 14.6 kDa (128aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4)
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 50pg/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human GM-CSF in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 50pg/ml.
Related Publications
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Poudel B, et al. Collagen I enhances functional activities of human monocyte-derived dendritic cells via discoidin domain receptor 2. (Cell Immunol. 2012)
Kusakabe Y, et al. A standardized blood test for the routine clinical diagnosis of impaired GM-CSF signaling using flow cytometry. (J Immunol Methods. 2014)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.