NKMAX

Product Information

Product Type

Recombinant Protein

Expression System

E.coli

Domain

1-205aa

UniProt No.

P04792

NCBI Accession No.

NP_001531

Alternative names

Heat shock 27 kDa protein, Hspb1, Hsp25, Hsp27, Hsp27, HspB1, Heat shock 27 kDa protein, HSP 27, Growth-related 25 kDa protein, P25, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, at shock 25kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat Shock Protein27, HS.76067, Hsp 28, Hsp B1, Hsp28, HspB1, SRP27, Stress responsive protein 27.

Product Specification

Molecular Weight

22.7 kDa (205aa) confirmed by MALDI-TOF

Concentration

1mg/ml (determined by Bradford assay)

Formulation

Liquid in. 20mM HEPES buffer (pH 7.4) containing 100mM KCl, 1mM DTT

Purity

> 95% by SDS-PAGE

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

Non-Tagged

Applications

SDS-PAGE

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

Magnify

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Related Publications

Kaiser F, et al. Monocyte cytokine synthesis in response to extracellular cell stress proteins suggests these proteins exhibit network behaviour. (Cell Stress Chaperones. 2014)
Casola C, et al. S100 alone has the same destructive effect on retinal ganglion cells as in combination with HSP 27 in an autoimmune glaucoma model. (J Mol Neurosci. 2015)
Casola C, et al. Specific Inner Retinal Layer Cell Damage in an Autoimmune Glaucoma Model Is Induced by GDNF With or Without HSP27. (Invest Ophthalmol Vis Sci. 2016)
Reinehr S, et al. HSP27 immunization reinforces AII amacrine cell and synapse damage induced by S100 in an autoimmune glaucoma model. (Cell Tissue Res. 2018)
Kaiser F, et al. Association between circulating levels of heat-shock protein 27 and aggressive periodontitis. (Cell Stress Chaperones. 2018)
Grotegut P, et al. Destructive Effect of Intravitreal Heat Shock Protein 27 Application on Retinal Ganglion Cells and Neurofilament. (Int J Mol Sci. 2020)
Eickholz P, et al. Effect of nonsurgical periodontal therapy on haematological parameters in grades B and C periodontitis: an exploratory analysis. (Clin Oral Investig. 2020)
Grotegut P, et al. Heat Shock Protein 27 Injection Leads to Caspase Activation in the Visual Pathway and Retinal T-Cell Response. (Int J Mol Sci. 2021)
Erb C, et al. HSP27 induced glaucomatous damage in mice of young and advanced age. (Front Cell Neurosci. 2023)
Joachim SC, Reinehr S. Does HSP27 injection induce glaucoma damage in mice? (Neural Regen Res. 2024)