Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-353aa
- UniProt No.
- Q99873
- NCBI Accession No.
- NP_938074.2
- Alternative names
- Protein arginine N-methyltransferase 1 isoform 3, HRMT1L2, HMT1 hnRNP methyltransferase-like 2 (S. cerevisiae), HCP1, ANM1, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4, IR1B4, ANM1, highly conserved protein 1
Product Specification
- Molecular Weight
- 84.2 kDa (750aa)
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 40mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 4mM MgCl₂, 2mM DTT, 40% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 10 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group on histone H4 per minute at 37C.
- Tag
- His-MBP-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
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Yamaguchi A, et al. The Effect of PRMT1-Mediated Arginine Methylation on the Subcellular Localization, Stress Granules, and Detergent-Insoluble Aggregates of FUS/TLS. (PLoS One. 2012)
Ryan VH, et al. Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation. (Mol Cell. 2018)
Dong R, et al. The translation initiating factor eIF4E and arginine methylation underlie G3BP1 function in dendritic spine development of neurons. (J Biol Chem. 2023)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.