NKMAX

Product Information

Product Type

Recombinant Protein

Expression System

Baculovirus

Domain

1-215aa

UniProt No.

P09429

NCBI Accession No.

NP_002119.1

Alternative names

Sulfoglucuronyl carbohydrate binding protein, SBP-1, HMGB1, HMG3, HMG1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin

Product Specification

Molecular Weight

25 kDa (223aa) confirmed by MALDI-TOF

Concentration

1mg/ml (determined by Bradford assay)

Formulation

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 0.5mM DTT, 10% glycerol

Purity

> 90% by SDS-PAGE

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

His-Tag

Applications

SDS-PAGE

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

Magnify

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Related Publications

Min HJ, et al. Chaperone-like Activity of High-Mobility Group Box 1 Protein and Its Role in Reducing the Formation of Polyglutamine Aggregates. (J Immunol. 2013)
Slean MM, et al. Interconverting Conformations of Slipped-DNA Junctions Formed by Trinucleotide Repeats Affect Repair Outcome. (Biochemistry. 2013)
Takahashi T, et al. Bone-Targeting Endogenous Secretory Receptor for Advanced Glycation End Products Rescues Rheumatoid Arthritis. (Mol Med. 2013)
Roy A, et al. Mast cell chymase degrades the alarmins heat shock protein 70, biglycan, HMGB1, and interleukin-33 (IL-33) and limits danger-induced inflammation. (J Biol Chem. 2014)
Sugimoto K, et al. Receptor for Advanced Glycation End Products - Membrane Type1 Matrix Metalloproteinase Axis Regulates Tissue Factor Expression via RhoA and Rac1 Activation in High-Mobility Group Box-1 Stimulated Endothelial Cells. (PLoS One. 2014)
Hernández-Pando R, et al. The Role of High Mobility Group Box 1 Protein (HMGB1) in the Immunopathology of Experimental Pulmonary Tuberculosis. (PLoS One. 2015)
Ito M, et al. High mobility group box 1 enhances hyperthermia-induced seizures and secondary epilepsy associated with prolonged hyperthermia-induced seizures in developing rats. (Metab Brain Dis. 2017)