Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 1-215aa
- UniProt No.
- P09429
- NCBI Accession No.
- NP_002119.1
- Alternative names
- Sulfoglucuronyl carbohydrate binding protein, SBP-1, HMGB1, HMG3, HMG1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin
Product Specification
- Molecular Weight
- 25 kDa (223aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 0.5mM DTT, 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
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Min HJ et al., Chaperone-like Activity of High-Mobility Group Box 1 Protein and Its Role in Reducing the Formation of Polyglutamine Aggregates. (J Immunol. 2013)
Slean MM et al., Interconverting Conformations of Slipped-DNA Junctions Formed by Trinucleotide Repeats Affect Repair Outcome. (Biochemistry. 2013)
Takahashi T et al., Bone-Targeting Endogenous Secretory Receptor for Advanced Glycation End Products Rescues Rheumatoid Arthritis. (Mol Med. 2013)
Roy A et al., Mast cell chymase degrades the alarmins heat shock protein 70, biglycan, HMGB1, and interleukin-33 (IL-33) and limits danger-induced inflammation. (J Biol Chem. 2014)
Sugimoto K et al., Receptor for Advanced Glycation End Products - Membrane Type1 Matrix Metalloproteinase Axis Regulates Tissue Factor Expression via RhoA and Rac1 Activation in High-Mobility Group Box-1 Stimulated Endothelial Cells. (PLoS One. 2014)
Hernández-Pando R et al. The Role of High Mobility Group Box 1 Protein (HMGB1) in the Immunopathology of Experimental Pulmonary Tuberculosis. (PLoS One. 2015)
Ito M et al., High mobility group box 1 enhances hyperthermia-induced seizures and secondary epilepsy associated with prolonged hyperthermia-induced seizures in developing rats. (Metab Brain Dis. 2017)
Jang J et al., Recombinant RAGE antagonist peptide promotes alveolar epithelial cell regeneration via the RAGE/MAPKs/MMP2 pathway in emphysema. (Biochem Pharmacol. 2025)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.