NKMAX

Product Information

Product Type

Recombinant Protein

Expression System

E.coli

Domain

1-154aa

UniProt No.

P00441

NCBI Accession No.

NP_000445.1

Alternative names

Superoxide dismutase 1 soluble, ALS, SOD, ALS1, IPOA, Cu-Zn superoxide dismutase, Superoxide dismutase 1 soluble, SOD1, Superoxide dismutase 1, soluble ALS 1, Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult, Cu/Zn SOD, Cu/Zn superoxide dismutase, Homodimer, Indophenoloxidase A, Mn superoxide dismutase, SOD 1, SOD soluble, SOD2, SODC, Superoxide dismutase 1 soluble, Superoxide dismutase Cu Zn, Superoxide dismutase cystolic,

Product Specification

Molecular Weight

15.9 kDa (154aa) confirmed by MALDI-TOF

Concentration

1mg/ml (determined by Bradford assay)

Formulation

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 500unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

Magnify

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Related Publications

Kwon MJ, et al. Superoxide Dismutase 3 Controls Adaptive Immune Responses and Contributes to the Inhibition of Ovalbumin-Induced Allergic Airway Inflammation in Mice. (Antioxid Redox Signal. 2012)
Kwon MJ, et al. Superoxide dismutase 3 suppresses hyaluronic acid fragments mediated skin inflammation by inhibition of toll-like receptor 4 signaling pathway: superoxide dismutase 3 inhibits reactive oxygen species-induced trafficking of toll-like receptor 4 to lipid rafts. (Antioxid Redox Signal. 2012)