Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-316aa
- UniProt No.
- P15121
- NCBI Accession No.
- NP_001619.1
- Alternative names
- Aldo-keto reductase family1 member B1, AKR1B1, ADR, ALDR1, ALR2, AR, Aldose reductase, AKR1B 1, Aldehyde reductase, aldr 1, aldr-1, Lii5-2 CTCL tumor antigen, low Km aldose reductase
Product Specification
- Molecular Weight
- 35.8 kDa (316aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is >800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
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Fatmawati S, et al. Ganoderic acid Df, a new triterpenoid with aldose reductase inhibitory activity from the fruiting body of Ganoderma lucidum. (Fitoterapia. 2010)
Onoda T, et al. Inhibitory activities of selected Kampo formulations on human aldose reductase. (BMC Complement Altern Med. 2014)
Kuroda M, et al. Chemical Constituents of the Leaves of Tussilago farfara and their Aldose Reductase Inhibitory Activity. (Nat Prod Commun. 2016)
Ishibashi F, et al. Synthesis and structure-activity relationship study of aldose reductase inhibiting marine alkaloid lukianol A and its derivatives. (Biosci Biotechnol Biochem. 2022)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.