NKMAX

Product Information

Product Type

Recombinant Protein

Expression System

E.coli

Domain

1-316aa

UniProt No.

P15121

NCBI Accession No.

NP_001619.1

Alternative names

Aldo-keto reductase family1 member B1, AKR1B1, ADR, ALDR1, ALR2, AR, Aldose reductase, AKR1B 1, Aldehyde reductase, aldr 1, aldr-1, Lii5-2 CTCL tumor antigen, low Km aldose reductase

Product Specification

Molecular Weight

35.8 kDa (316aa) confirmed by MALDI-TOF

Concentration

1mg/ml (determined by Bradford assay)

Formulation

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >800pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37C.

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

SDS-PAGE

Magnify

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Related Publications

Fatmawati S et al., Ganoderic acid Df, a new triterpenoid with aldose reductase inhibitory activity from the fruiting body of Ganoderma lucidum. (Fitoterapia. 2010)
Onoda T et al., Inhibitory activities of selected Kampo formulations on human aldose reductase. (BMC Complement Altern Med. 2014)
Kuroda M et al., Chemical Constituents of the Leaves of Tussilago farfara and their Aldose Reductase Inhibitory Activity. (Nat Prod Commun. 2016)
Ishibashi F et al., Synthesis and structure-activity relationship study of aldose reductase inhibiting marine alkaloid lukianol A and its derivatives. (Biosci Biotechnol Biochem. 2022)
Matsuo Y et al., Lanceolanone A, a new biflavanone, and a chalcone glucoside from the flower heads of Coreopsis lanceolata and their aldose reductase inhibitory activity and AMPK activation. (J Nat Med. 2023)
Gheorghe D et al., THE IN-SILICO OPTIMIZATION OF A BATCH REACTOR IN THE 2ND STEP OF CETUS PROCESS FOR D-FRUCTOSE PRODUCTION. (U.P.B. Sci. Bull., Series B. 2025)