Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-434aa
- UniProt No.
- P06733
- NCBI Accession No.
- NP_001419.1
- Alternative names
- Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, PPH, Plasminogen-binding protein, ENO1L1, MBPB1, MPB1, ENO1-IT1, ENO1 intronic transcript 1
Product Specification
- Molecular Weight
- 47.1 kDa (434aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM MgSO4, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 20,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
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Chen X, et al. Interaction between granulin A and enolase 1 attenuates the migration and invasion of human hepatoma cells. (Oncotarget. 2017)
Hashimoto Y, et al. Comparison of clinical characteristics in patients with Vogt-Koyanagi-Harada disease with and without anti-retinal antibodies. (Graefes Arch Clin Exp Ophthalmol. 2019)
Hirakata T, et al. Acute unilateral inner retinal dysfunction with photophobia: importance of electrodiagnosis. (Jpn J Ophthalmol. 2020)
Hashimoto Y, et al. Comparison of clinical characteristics in patients with acute zonal occult outer retinopathy according to anti-retinal antibody status. (Graefes Arch Clin Exp Ophthalmol. 2021)
Inoue Y, et al. α-Enolase reduces cerebrovascular Aβ deposits by protecting Aβ amyloid formation. (Cell Mol Life Sci. 2022)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.