Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-364aa
- UniProt No.
- P04075
- NCBI Accession No.
- NP_908930
- Alternative names
- Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDA
Product Specification
- Molecular Weight
- 41.5 kDa (384aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 10unit/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3-phosphate per minute at pH 7.5 at 37C.
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
-
Jung SH, et al. Integrative Proteomic Profiling of Protein Activity and Interactions Using Protein Arrays. (Mol Cell Proteomics. 2012)
Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016)
Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.