Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 25-222aa
- UniProt No.
- P04179
- NCBI Accession No.
- NP_001019636.1
- Alternative names
- Superoxide dismutase [Mn] mitochondrial, IPOB, MNSOD, MVCD6, Superoxide dismutase [Mn], mitochondrial IPO B, Manganese SOD, Manganese superoxide dismutase, Mn SOD, SOD 2, SOD2, Superoxide dismutase [Mn] mitochondrial, Superoxide dismutase 2 mitochondrial, Superoxide Dismutase-2
Product Specification
- Molecular Weight
- 24.4 kDa (219aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 1,000unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
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Kwon MJ, et al. Superoxide dismutase 3 suppresses hyaluronic acid fragments mediated skin inflammation by inhibition of toll-like receptor 4 signaling pathway: superoxide dismutase 3 inhibits reactive oxygen species-induced trafficking of toll-like receptor 4 to lipid rafts. (Antioxid Redox Signal. 2012)
Kwon MJ, et al. Superoxide dismutase 3 controls adaptive immune responses and contributes to the inhibition of ovalbumin-induced allergic airway inflammation in mice. (Antioxid Redox Signal. 2012)
Peskin AV, Winterbourn CC. Assay of superoxide dismutase activity in a plate assay using WST-1. (Free Radic Biol Med. 2017)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.