Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-227aa
- UniProt No.
- P22061
- NCBI Accession No.
- AAH08748.1
- Alternative names
- Protein-L-isoaspartate D-aspartate O-methyltransferase, PIMT, L-isoaspartyl protein carboxyl methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase
Product Specification
- Molecular Weight
- 28.7 kDa (263aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
- Purity
- > 95% by SDS-PAGE
- Tag
- His-Tag
- Applications
- SDS-PAGE
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Related Publications
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Maeda H, et al. Rapid survey of four Asp isomers in disease-related proteins by LC-MS combined with commercial enzymes. (Anal Chem. 2015)
Lyon YA, et al. Differences in α-Crystallin isomerization reveal the activity of protein isoaspartyl methyltransferase (PIMT) in the nucleus and cortex of human lenses. (Exp Eye Res. 2018)
Takata T, et al. Site-specific rapid deamidation and isomerization in human lens αA-crystallin in vitro. (Protein Sci. 2020)
Magami K, et al. Isomerization of Asp is essential for assembly of amyloid-like fibrils of αA-crystallin-derived peptide. (PLoS One. 2021)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.