Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-289aa
- UniProt No.
- P00491
- NCBI Accession No.
- NP_000261
- Alternative names
- Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PuNP
Product Specification
- Molecular Weight
- 34.2 kDa (309aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 120unit/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 umole of inosine with inorganic phosphate per minute at pH 7.5 at 25C.
- Tag
- His-Tag
- Applications
- SDS-PAGE,Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.