Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-260aa
- UniProt No.
- Q16775
- NCBI Accession No.
- NP_001035517
- Alternative names
- Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1
Product Specification
- Molecular Weight
- 31.4 kDa (284aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 500unit/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37C.
- Tag
- His-Tag
- Applications
- Enzyme Activity,SDS-PAGE
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.