Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-450aa
- UniProt No.
- P06715
- NCBI Accession No.
- NP_417957
- Alternative names
- Glutathione oxidoreductase, ECK3485, gorA, JW3467
Product Specification
- Molecular Weight
- 51.2 kDa (473aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl,1mM DTT
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity is > 60unit/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.
- Tag
- His-Tag
- Applications
- Enzyme Activity,SDS-PAGE
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.