Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-184aa
- UniProt No.
- Q04760
- NCBI Accession No.
- NP_006699
- Alternative names
- Lactoylglutathione lyase, Glyoxalase I, Glx 1, GLO-1 Methylglyoxalase, Aldoketomutase
Product Specification
- Molecular Weight
- 20.7 kDa (184aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Specific activity: > 400unit/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- Enzyme Activity,SDS-PAGE
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.