Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 77-233aa
- UniProt No.
- P01375
- NCBI Accession No.
- NP_000585.2
- Alternative names
- Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF
Product Specification
- Molecular Weight
- 18.1 kDa (163aa)
- Concentration
- 1mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Biological Activity
Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
Related Publications
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Kim MJ et al., Cinnamomum verum extract as a potential therapeutic for atopic dermatitis through reducing lipid peroxidation and oxidative stress. (Front Pharmacol. 2026)
Jo HJ et al., Therapeutic Potential of Ruberythric Acid in Intestinal Inflammation and Barrier Function Reduction. (Prev Nutr Food Sci. 2026)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.