Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-413aa
- UniProt No.
- P17174
- NCBI Accession No.
- NP_002070
- Alternative names
- Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Aspartate transaminase 1, AST1, SGOT, AST, cAspAT, Cysteine aminotransferase cytoplasmic, Cysteine transaminase, Cytoplasmic, cCAT, Transaminase A
Product Specification
- Molecular Weight
- 48.4 kDa (433aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Specific activity is > 50unit/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.