Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 26-173aa
- UniProt No.
- P25445
- NCBI Accession No.
- NP_000034
- Alternative names
- Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, FAS1, APT1, TNFRSF6
Product Specification
- Molecular Weight
- 17.7 kDa (156aa)
- Concentration
- 0.5mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured by ability to inhibit FAS ligand-induced apoptosis assay using Jurkat human acute T cell leukemia cells in the presence of 2ng/ml of human FAS ligand. The ED50 range ≤ 200ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human CD95/FAS inhibit apoptosis of the Jurkat human acute T cell leukemia cells in the presence of human FAS ligand. The ED50 range ≤ 200 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.