Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 23-202aa
- UniProt No.
- P15018
- NCBI Accession No.
- NP_002300
- Alternative names
- Leukemia inhibitory factor isoform1, LIF, CDF, DIA, HILDA, MLPLI
Product Specification
- Molecular Weight
- 20.8 kDa (189aa)
- Concentration
- 0.25mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.5 ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Biological Activity
Human LIF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 0.5ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.