Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 1-531aa
- UniProt No.
- P52480
- NCBI Accession No.
- NP_035229
- Alternative names
- Pyruvate kinase PKM isoform M2, Pyruvate kinase muscle isozyme, pyruvate kinase M1/2, Threonine-protein kinase PKM2, Tyrosine-protein kinase PKM2, Pk3, Pkm2, Pykm
Product Specification
- Molecular Weight
- 60.2 kDa (554aa) Confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH8.5) containing 0.2M NaCl, 1mM DTT, 30% glycerol
- Purity
- > 85% by SDS-PAGE
- Bioactivity
- Specific activity: > 50,000pmol/min/ug. One unit will convert 1.0pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C
- Tag
- His-Tag
- Applications
- SDS-PAGE, Enzyme Activity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.