Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 977-1029aa
- UniProt No.
- P01132
- NCBI Accession No.
- NP_034243
- Alternative names
- Epidermal growth factor, Pro-epidermal growth factor isoform1, AI790464
- Additional Information
- ATGP1532 has been replaced with a catalog number ATGP3729.
Product Specification
- Molecular Weight
- 8.6 kDa (77aa) Confirmed by MALDI-TOF
- Concentration
- 0.25mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range ≤ 1ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Mouse EGF in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range ≤ 1 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.