Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 80-235aa
- UniProt No.
- P16599
- NCBI Accession No.
- NP_036807
- Alternative names
- Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF
Product Specification
- Molecular Weight
- 19.9 kDa (181aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by absorbance at 280nm)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
- Purity
- > 90% by SDS-PAGE
- Bioactivity
- Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Rat TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.