Product Information
- Product Type
- Recombinant Protein
- Expression System
- Baculovirus
- Domain
- 23-335aa(p40)/23-215aa(p35)
- UniProt No.
- P43432(p40)/P43431(p35)
- NCBI Accession No.
- NP_001290173(p40)/NP_032377(p35)
- Alternative names
- IL12A, IL12B, CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Interleukin-12 alpha chain, Interleukin-12 beta chain, NK cell stimulatory factor chain 1, p35, NK cell stimulatory factor chain 2, p40, NKSF1, NKSF2
Product Specification
- Molecular Weight
- 35.7 kDa (p40, 313aa)
22.5 kDa (p35, 199aa)
The heterodimeric Il12 has a predicted Molecular weight of 58.3 kDa (512aa).
Molecular weight on SDS-PAGE will appear higher
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 for this effect is less or equal to 10ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE visualized by coomassie blue stain.
Lane 1: reducing condition
Lane 2: non-reducing condition
Lane 1: reducing condition
Lane 2: non-reducing condition
Biological Activity
Mouse IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range is less or equal to 10 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.