Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 27-178aa
- UniProt No.
- P25085
- NCBI Accession No.
- NP_001034790
- Alternative names
- Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, IL1RA
- Additional Information
- ATGP1516 has been replaced with a catalog number ATGP3884.
Product Specification
- Molecular Weight
- 20 kDa (177aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 60ng/ml with Mouse IL-1 alpha.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Biological Activity
Mouse IL-1ra/IL-1F3 inhibits Mouse IL-1 alpha induced cell proliferation in the D10.G4.1 mouse helper T cell. The ED50 range is less or equal to 60 ng/ml.
Related Publications
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Hiramoto K, et al. The role of the active oxygen produced from Gp91phox NADPH oxidase on the newborn weight of mouse pups. (Biology and Medicine. 2015)
Hiramoto K, et al. Gp91phox NADPH oxidase modulates litter size by regulating mucin1 in the uterus of mice. (Syst Biol Reprod Med. 2017)
Hiramoto K, et al. The Role of gp91phox and the Effect of Tranexamic Acid Administration on Hair Color in Mice. (Int J Mol Sci. 2019)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.