Product Information
- Product Type
- Recombinant Protein
- Expression System
- HEK293
- Domain
- 18-740aa
- UniProt No.
- Q9BYF1
- NCBI Accession No.
- NP_068576.1
- Alternative names
- Angiotensin-converting enzyme 2, ACEH, Angiotensin-converting enzyme-related carboxypeptidase, ACE-related carboxypeptidase, Metalloprotease MPROT15, ACE2
Product Specification
- Molecular Weight
- 110.8kDa (965aa)
- Concentration
- 0.5mg/ml (determined by Absorbance at 280nm)
- Formulation
- Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
- Purity
- > 90% by SDS - PAGE
- Bioactivity
- Measured by its binding ability in a functional ELISA with SARS-CoV-2 Spike RBD. The ED50 range ≤ 100ng/ml. Specific activity is > 250 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK(Dnp)-OH per minute at pH 7.5, at 25C.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- hIgG-His-Tag
- Applications
- SDS-PAGE, Enzyme Activity, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
Biological Activity
SARS-CoV-2 Spike RBD is coated at 5 ug/ml (100 ul/well) can bind Human ACE-2. The ED50 range ≤ 100ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.