Product Information
- Product Type
- Recombinant Protein
- Expression System
- E.coli
- Domain
- 113-261aa
- UniProt No.
- P29965
- NCBI Accession No.
- NP_000065
- Alternative names
- CD40 ligand, CD40-L, CD40L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, TNFSF5, CD154, hyper-IgM syndrome, HIGM1, T-B cell-activating molecule, T-BAM
Product Specification
- Molecular Weight
- 18.3 kDa (169aa) confirmed by MALDI-TOF
- Concentration
- 0.5mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured by its binding ability in a functional ELISA with Human CD40/TNFRSF5 (CAT# ATGP4007).
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- His-Tag
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human CD40 Ligand/TNFSF5 is coated at 5 ug/ml (100 ul/well) can bind Human CD40/TNFRSF5 (CAT# ATGP4007) in a Functional ELISA assay.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.