NKMAX

Product Information

Product Type

Monoclonal antibody

Clone Number

4C2

UniProt No.

Q53EL6

NCBI Accession No.

NP_055271

Alternative names

Programmed cell death 4 isoform 1, neoplastic transformation inhibitor, H731, MGC33046, MGC33047, Programmed cell death 4 isoform 1, PDPC4, Programmed cell death 4 isoform 1, PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), nuclear antigen H731

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human PDCD4 (1-469aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB, ICC/IF, FACS

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with ATGA0601 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: K562 cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: 293T cell lysate

Flow cytometry (FACS)

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Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with ATGA0601 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).