NKMAX

Product Information

Product Type

Monoclonal antibody

Clone Number

AT18D10

UniProt No.

K0BRG7

NCBI Accession No.

AFS88936

Alternative names

Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERS-CoV RBD, MERS RBD, receptor binding domain, RBD, Spike RBD protein

Product Specification

Host

Mouse

Reacts With

MERS-CoV

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant MERS-CoV Spike RBD (358-606aa) purified from Baculovirus

Isotype

IgG1 kappa

Purification

By protein-A affinity chromatography

Applications

ELISA, WB

Usage

The antibody has been tested by ELISA analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

Magnify

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MERS Spike RBD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant MERS Spike protein (ATGP3979)
Lane 2.: Recombinant MERS Spike S1 protein (ATGP3980)
Lane 3.: Recombinant MERS Spike S2 protein (ATGP3981)
Lane 4.: Recombinant MERS Spike RBD protein (ATGP3982)

Additional Information

Magnify

ELISA: MERS Spike RBD Antibody (1ug/ml) specifically recognizes MERS Spike RBD recombinant protein, but not interacted MERS Spike, Spike S1 and Spike S2 recombinant protein recombinant protein in ELISA.

Related Publications

Lee K, et al. Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus. (Microbiol Immunol. 2018)
Goo J, et al. Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. (Virus Res. 2020)