Product Information
- Product Type
- Recombinant Animal Free Protein
- Expression System
- E.coli
- Domain
- 971-1023aa
- UniProt No.
- P01133
- NCBI Accession No.
- NP_001954.2
- Alternative names
- Epidermal growth factor, Pro-epidermal growth factor, urogastrone, EGF
Product Specification
- Molecular Weight
- 6.3 kDa (54aa) confirmed by MALDI-TOF
- Concentration
- 1mg/ml (determined by Bradford assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4)
- Purity
- > 95% by SDS-PAGE
- Bioactivity
- Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 0.1 ng/ml.
- Endotoxin
- < 1 EU per 1ug of protein (determined by LAL method)
- Tag
- Non-Tagged
- Applications
- SDS-PAGE, Bioactivity
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
SDS-PAGE
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Biological Activity
Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 0.1 ng/ml.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.