Recombinant human TRANCE/RANK L/TNFSF11 protein

Cat No.
ATGP4157

Size

QTY

List Price

10ug

USD 150


20ug

USD 260

50ug

USD 430

100ug

USD 720

250ug

USD 1,260

500ug

USD 2,160

USD 0



Product Information

Product Type
Recombinant Proteins
Expression System
E.coli
Domain
140-317aa
UniProt No.
O14788
NCBI Accession No.
NP_003692.1
Alternative names
Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254

Product Specification

Molecular Weight
22.3 kDa (199aa) confirmed by MALDI-TOF
Concentration
0.5mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Purity
> 85% by SDS – PAGE
Bioactivity
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤ 25 ng/ml.
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles

Data

SDS-PAGE

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Biological Activity

Human TRANCE stimulates osteoclast differentiation in the RAW 264.7 mouse monocyte/macrophage cells. The ED50 range ≤ 25 ng/ml.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.