Human PP2C alpha /PPM1A antibody

Cat No.
APP0401

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
p6c7
UniProt No.
P35813
NCBI Accession No.
NP_066283
Alternative names
Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, PDP1, PDP, PPM2C, PP2C alpha, Protein phosphatase 2C, PDP 1, EC 3.1.3.43, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, PPM1A, Protein phosphatase 1A (formerly 2C), ppm1a, FLJ42306, MGC9201, PP2CA, PP2Calpha, PP2C-alpha, protein phosphatase Mg2+/Mn2+ dependent 1A

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PP2Calpha (1-382aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtainoptimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of PP2C alpha in HeLa cells. The cell was stained with APP0401 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: K562 cell lysate
Lane 4.: MCF7 cell lysate
Lane 5.: A549 cell lysate
Lane 6.: Raji cell lysate
The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse kidney tissue lysate
Lane 2.: Mouse brain tissue lysate
Lane 3.: Mouse liver tissue lysate

Related Publications

    Kim YG, et al. Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase magnesium-dependent 1A autoantibodies in ankylosing spondylitis. Arthritis Rheumatol. 2014 Oct;66(10):2793-2803.
    Budhiraja S, et al. Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4T+ T cells and inhibits HIV-1 gene expression. Retrovirology. 2012 Jun 22;9:52.

    Heikkinen PT, et al. Hypoxia-activated Smad3-specific Dephosphorylation by PP2A. J Biol Chem. 2010 Feb 5;285(6):3740-9.

    Bu S, et al. Dihydrosphingosine 1-phosphate has a potent antifibrotic effect in scleroderma fibroblasts via normalization of phosphatase and tensin homolog levels. Arthritis Rheum. 2010 Jul;62(7):2117-26.

    Lin X, et al. PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 2006 Jun 2;125(5):915-28.

    Duan X, et al. Protein serine/threonine phosphatase PPM1A dephosphorylates Smad1 in the bone morphogenetic protein signaling pathway. J Biol Chem. 2006 Dec 1;281(48):36526-32.

    Bu S, et al. Opposite effects of dihydrosphingosine 1-phosphate and sphingosine 1-phosphate on transforming growth factor-beta/Smad signaling are mediated through the PTEN/PPM1A-dependent pathway. J Biol Chem. 2008 Jul 11;283(28):19593-602.

    Sun W, et al. PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced IKKbeta-NF-kappaB activation. Cell Signal. 2009 Jan;21(1):95-102.

    Shukla A, et al. TGF-beta signalling is regulated by Schnurri-2-dependent nuclear translocation of CLIC4 and consequent stabilization of phospho-Smad2 and 3. Nat Cell Biol. 2009 Jun;11(6):777-84.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.