NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

p6c7

UniProt No.

P35813

NCBI Accession No.

NP_066283

Alternative names

Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, PDP1, PDP, PPM2C, PP2C alpha, Protein phosphatase 2C, PDP 1, EC 3.1.3.43, Pyruvate dehydrogenase phosphatase catalytic subunit 1, PDPC 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, PPM1A, Protein phosphatase 1A (formerly 2C), ppm1a, FLJ42306, MGC9201, PP2CA, PP2Calpha, PP2C-alpha, protein phosphatase Mg2+/Mn2+ dependent 1A

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human PP2Calpha (1-382aa) purified from E. coli

Isotype

IgG2b kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtainoptimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

Magnify

ICC/IF analysis of PP2C alpha in HeLa cells. The cell was stained with APP0401 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: K562 cell lysate
Lane 4.: MCF7 cell lysate
Lane 5.: A549 cell lysate
Lane 6.: Raji cell lysate

Magnify

The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse kidney tissue lysate
Lane 2.: Mouse brain tissue lysate
Lane 3.: Mouse liver tissue lysate

Related Publications

Kim YG, et al. Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase magnesium-dependent 1A autoantibodies in ankylosing spondylitis. Arthritis Rheumatol. 2014 Oct;66(10):2793-2803.
Budhiraja S, et al. Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4T+ T cells and inhibits HIV-1 gene expression. Retrovirology. 2012 Jun 22;9:52.
Heikkinen PT, et al. Hypoxia-activated Smad3-specific Dephosphorylation by PP2A. J Biol Chem. 2010 Feb 5;285(6):3740-9.
Bu S, et al. Dihydrosphingosine 1-phosphate has a potent antifibrotic effect in scleroderma fibroblasts via normalization of phosphatase and tensin homolog levels. Arthritis Rheum. 2010 Jul;62(7):2117-26.
Lin X, et al. PPM1A functions as a Smad phosphatase to terminate TGFbeta signaling. Cell. 2006 Jun 2;125(5):915-28.
Duan X, et al. Protein serine/threonine phosphatase PPM1A dephosphorylates Smad1 in the bone morphogenetic protein signaling pathway. J Biol Chem. 2006 Dec 1;281(48):36526-32.
Bu S, et al. Opposite effects of dihydrosphingosine 1-phosphate and sphingosine 1-phosphate on transforming growth factor-beta/Smad signaling are mediated through the PTEN/PPM1A-dependent pathway. J Biol Chem. 2008 Jul 11;283(28):19593-602.
Sun W, et al. PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced IKKbeta-NF-kappaB activation. Cell Signal. 2009 Jan;21(1):95-102.
Shukla A, et al. TGF-beta signalling is regulated by Schnurri-2-dependent nuclear translocation of CLIC4 and consequent stabilization of phospho-Smad2 and 3. Nat Cell Biol. 2009 Jun;11(6):777-84.