Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- p6c7
- UniProt No.
- P35813
- NCBI Accession No.
- NP_066283
- Alternative names
- Pyruvate dehydrogenase phosphatase catalytic subunit 1, Pyruvate dehydrogenase acetyl-transferring-phosphatase 1, Protein phosphatase Mg2+/Mn2+dependent 1A, Protein phosphatase 2C, Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform, Protein phosphatase 1A (formerly 2C), PPM2C, PPM1A, PP2CA, PP2C alpha, PDPC 1, PDP 1, PDP, MGC9201, FLJ42306, EC 3.1.3.43
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human PP2Calpha (1-382aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF
- Usage
- The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtainoptimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of PP2C alpha in HeLa cells. The cell was stained with APP0401 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: K562 cell lysate
Lane 4.: MCF7 cell lysate
Lane 5.: A549 cell lysate
Lane 6.: Raji cell lysate
Lane 1.: Jurkat cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: K562 cell lysate
Lane 4.: MCF7 cell lysate
Lane 5.: A549 cell lysate
Lane 6.: Raji cell lysate
The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PP2C alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse kidney tissue lysate
Lane 2.: Mouse brain tissue lysate
Lane 3.: Mouse liver tissue lysate
Lane 1.: Mouse kidney tissue lysate
Lane 2.: Mouse brain tissue lysate
Lane 3.: Mouse liver tissue lysate
Related Publications
-
Kim JS, et al. The interaction of hepatitis B virus X protein and protein phosphatase type 2 Calpha and its effect on IL-6. (Biochem Biophys Res Commun. 2006)
Budhiraja S, et al. Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4(+) T cells and inhibits HIV-1 gene expression. Retrovirology. 2012
Kim YG, et al. Role of protein phosphatase magnesium-dependent 1A and anti-protein phosphatase magnesium-dependent 1A autoantibodies in ankylosing spondylitis. (Arthritis Rheumatol. 2014)
Tabony AM, et al. Protein phosphatase 2C-alpha knockdown reduces angiotensin II-mediated skeletal muscle wasting via restoration of mitochondrial recycling and function. (Skelet Muscle. 2014)
Chen W, et al. Ppm1b negatively regulates necroptosis through dephosphorylating Rip3. (Nat Cell Biol. 2015)
Pereira JM, et al. Infection Reveals a Modification of SIRT2 Critical for Chromatin Association. (Cell Rep. 2018)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.