- Product Type
- Monoclonal Antibody
- Clone Number
- UniProt No.
- NCBI Accession No.
- Alternative names
- HtrA serine peptidase 2, High temperature requirement protein A2, HtrA2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, PRSS25, OMI, PARK13
- Reacts With
- 1mg/ml (determined by BCA assay)
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Recombinant human HtrA2/Omi (134-458aa) purified from E. coli
- IgG1 kappa
- By protein-G affinity chromatography
- ELISA, WB
- The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Western blot analysis (WB)
The cell lysates of 293T, MCF7, HT-29, HL-60, HeLa and tissue lysates of mouse brain, rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Kim GY, et al. A simple and rapid strategy for the molecular cloning and monitoring of mouse HtrA2 serine protease. (Biotechnol Lett. 2008)
Park HJ, et al. Intracellular Abeta and C99 aggregates induce mitochondria-dependent cell death in human neuroglioma H4 cells through recruitment of the 20S proteasome subunits. (Brain Res. 2009)
Shin EJ, et al. Matrix metalloproteinase-3 is activated by HtrA2/Omi in dopaminergic cells: relevance to Parkinsons disease. (Neurochem Int. 2012)
Goo HG, et al. HtrA2/Omi deficiency causes damage and mutation of mitochondrial DNA. (Biochim Biophys Acta. 2013)
Goo HG, et al. HtrA2/Omi influences the stability of LON protease 1 and prohibitin, proteins involved in mitochondrial homeostasis. (Exp Cell Res. 2014)
Nam MK, et al. HtrA2 regulates α-Synuclein-mediated mitochondrial reactive oxygen species production in the mitochondria of microglia. (Biochem Biophys Res Commun. 2023)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.