- Product Type
- Monoclonal Antibody
- Clone Number
- UniProt No.
- NCBI Accession No.
- Alternative names
- interferon regulatory factor 5, irf5
- Reacts With
- 1mg/ml (determined by BCA assay)
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Recombinant human IRF-5 (176-240aa) purified from E. coli
- IgG1 kappa
- By protein-A affinity chromatography
- ELISA, WB, ICC/IF, IHC, FACS
- The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
ICC/IF analysis of IRF-5 in THP-1 cells. The cell was stained with AIR0611 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of IRF-5 in Raw264.7 cells. The cell was stained with AIR0611 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western blot analysis (WB)
The cell lysates of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Flow cytometry (FACS)
Flow cytometry analysis of IRF5 in THP-1 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
IHC Comment And Pic
Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
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Chionh YT, et al. Protease-activated receptor 1 suppresses Helicobacter pylori gastritis via the inhibition of macrophage cytokine secretion and interferon regulatory factor 5. Mucosal Immunol. 2015 Jan;8(1):68-79.
Li D, et al. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. Sci Rep. 2016 Aug 2;6:31002.
O Carroll C, et al. Identification of a unique hybrid macrophage-polarization state following recovery from lipopolysaccharide tolerance. J Immunol. 2014 Jan 1;192(1):427-36.
Yeon SI, et al. Development of monoclonal antibodies against human IRF-5 and their use in identifying the binding of IRF-5 to nuclear import proteins karyopherin-alpha1 and -beta1. Yonsei Med J. 2008 Dec 31;49(6):1023-31.
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.