Human IRF-5 antibody

Cat No.
AIR0611

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
10T1
UniProt No.
Q13568
NCBI Accession No.
NP_116032.1
Alternative names
interferon regulatory factor 5, irf5

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human IRF-5 (176-240aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF, IHC, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of IRF-5 in THP-1 cells. The cell was stained with AIR0611 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of IRF-5 in Raw264.7 cells. The cell was stained with AIR0611 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF-5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Flow cytometry (FACS)

Flow cytometry analysis of IRF5 in THP-1 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

IHC Comment And Pic

Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Related Publications

    He H, et al. Immobilized heavy chain-hyaluronic acid polarizes lipopolysaccharide-activated macrophages toward M2 phenotype. J Biol Chem. 2013 Sep 6;288(36):25792-803.
    Pandey AK, et al. NOD2, RIP2 and IRF5 play a critical role in the type I interferon response to Mycobacterium tuberculosis. PLoS Pathog. 2009 Jul;5(7):e1000500.

    Watanabe T, et al. Muramyl dipeptide activation of nucleotide-binding oligomerization domain 2 protects mice from experimental colitis. J Clin Invest. 2008 Feb;118(2):545-59.

    Ye L, et al. Methamphetamine enhances Hepatitis C virus replication in human hepatocytes. J Viral Hepat. 2008 Apr;15(4):261-70.

    Polioudakis D, et al. A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes. Nucleic Acids Res. 2013 Feb 1;41(4):2239-54.

    Chionh YT, et al. Protease-activated receptor 1 suppresses Helicobacter pylori gastritis via the inhibition of macrophage cytokine secretion and interferon regulatory factor 5. Mucosal Immunol. 2015 Jan;8(1):68-79.

    Li D, et al. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. Sci Rep. 2016 Aug 2;6:31002.

    O Carroll C, et al. Identification of a unique hybrid macrophage-polarization state following recovery from lipopolysaccharide tolerance. J Immunol. 2014 Jan 1;192(1):427-36.

    Yeon SI, et al. Development of monoclonal antibodies against human IRF-5 and their use in identifying the binding of IRF-5 to nuclear import proteins karyopherin-alpha1 and -beta1. Yonsei Med J. 2008 Dec 31;49(6):1023-31.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.