NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

5H7

UniProt No.

Q15848

NCBI Accession No.

NP_004788

Alternative names

ADIPOQ, ACDC, ACRP30, APM1, GBP28, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human adiponectin (15-244aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF

Usage

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

Magnify

ICC/IF analysis of Adiponectin in Hep3B cells. The cell was stained with AAD0614 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

Magnify

The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : 293T cell lysate
Lane 2. : Adiponectin collagen domain(15-107aa) transfected 293T cell lysate
Lane 3. : Adiponectin Full domain(15-244aa) transfected 293T cell lysate
Lane 4. : Adiponectin globular domain(108-244aa) transfected 293T cell lysate

Related Publications

Almer G, et al. Adiponectin-coated nanoparticles for enhanced imaging of atherosclerotic plaques. (Int J Nanomedicine. 2011)
Almer G, et al. Globular domain of adiponectin: promising target molecule for detection of atherosclerotic lesions. (Biologics. 2011)