Human KIR2DL1/CD158a antibody

Cat No.
AKR0620

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
2F9
UniProt No.
P43626
NCBI Accession No.
NP_055033
Alternative names
Killer cell immunoglobulin-like receptor 2DL1, KIR2DL1, CD158A, NKAT1, KIR2DL1 (p58 KIR, CD158), MHC class I NK cell receptor, Natural killer-associated transcript 1, NKAT-1, p58 natural killer cell receptor clones CL-42/47.11, p58 NK receptor, p58.1 MHC class-I-specific NK receptor, Killer cell immunoglobulin-like receptor 2DL1, Killer cell immunoglobulin-like receptor 2DL1 Killer cell immunoglobulin like receptor 2DL1, MHC class I NK cell receptor, Natural killer associated transcript 1, NKAT 1, p58 NK receptor, p58.1 MHC class I specific NK receptor

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human kIR2DL1 (23-223 aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of KIR2DL1 in Hep3B cells. The cell was stained with AKR0620 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: Jurkat cell lysate
The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR2DL1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: KIR2DL1 recombinant protein
Lane 2.: KIR2DL3 recombinant protein
Lane 3.: KIR2DS4 recombinant protein
Lane 4.: KIR2DL4 recombinant protein

Flow cytometry (FACS)

Flow cytometry analysis of KIR2DL1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.