Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 4G2
- UniProt No.
- P07339
- NCBI Accession No.
- NP_001900
- Alternative names
- CTSD, CPSD, Cathepsin D, Lysosomal aspartyl protease, CLN10, Ceroid-lipofuscinosis, Neuronal 10
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human Cathepsin D (21-412 aa) purified from E. coli
- Isotype
- IgG2b kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, IHC
- Usage
- The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Western blot analysis (WB)
The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: Hep3B cell lysate
Lane 3.: MDA-MB-231 cell lysate
Lane 4.: MCF7 cell lysate
Lane 1.: HepG2 cell lysate
Lane 2.: Hep3B cell lysate
Lane 3.: MDA-MB-231 cell lysate
Lane 4.: MCF7 cell lysate
The recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombinant Human CTSB
Lane 2. : Recombinant Human CTSD
Lane 3. : Recombinant Human CTSE
Lane 4. : Recombinant Human CTSF
Lane 5. : Recombinant Human CTSH
Lane 6. : Recombinant Human CTSK
Lane 7. : Recombinant Human CTSL
Lane 8. : Recombinant Human CTSS
Lane 9. : Recombinant Human CTSW
Lane 10. : Recombinant Human CTSZ
Lane 1. : Recombinant Human CTSB
Lane 2. : Recombinant Human CTSD
Lane 3. : Recombinant Human CTSE
Lane 4. : Recombinant Human CTSF
Lane 5. : Recombinant Human CTSH
Lane 6. : Recombinant Human CTSK
Lane 7. : Recombinant Human CTSL
Lane 8. : Recombinant Human CTSS
Lane 9. : Recombinant Human CTSW
Lane 10. : Recombinant Human CTSZ
IHC Comment And Pic
Paraffin embedded sections of human breast lobule tissue were incubated with anti-human Cathepsin D (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.