Human Cathepsin D antibody

Cat No.
ACH0627

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
4G2
UniProt No.
P07339
NCBI Accession No.
NP_001900
Alternative names
CTSD,CPSD, CLN10, MGC2311, cathepsin D, cathepsin D (lysosomal aspartyl protease), ceroid-lipofuscinosis, neuronal 10

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Cathepsin D (21-412 aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, IHC
Usage
The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: Hep3B cell lysate
Lane 3.: MDA-MB-231 cell lysate
Lane 4.: MCF7 cell lysate
The recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cathepsin D antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1. : Recombinant Human CTSB
Lane 2. : Recombinant Human CTSD
Lane 3. : Recombinant Human CTSE
Lane 4. : Recombinant Human CTSF
Lane 5. : Recombinant Human CTSH
Lane 6. : Recombinant Human CTSK
Lane 7. : Recombinant Human CTSL
Lane 8. : Recombinant Human CTSS
Lane 9. : Recombinant Human CTSW
Lane 10. : Recombinant Human CTSZ

IHC Comment And Pic

Paraffin embedded sections of human breast lobule tissue were incubated with anti-human Cathepsin D (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.