Human alpha, Beta -Synuclein antibody

Cat No.
ASC0632

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
3B6
UniProt No.
P37840, Q16143
NCBI Accession No.
NP_000336, NP_003076
Alternative names
a, b - synuclein, Alpha synuclein, Beta synuclein, NACP, Non A beta component of AD amyloid, Non A4 component of amyloid precursor, PARK1, PARK4, Parkinson disease familial 1, PD1, SNCA, SNCB, SNCB protein, Synuclein alpha, Synuclein beta

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human a-synuclein (119-140aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF,FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of alpha, beta-Synuclein in LNCaP cells. The cell was stained with ACS0632 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of alpha, beta-Synuclein in U87MG cells. The cell was stained with ACS0632 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of alpha, beta-Synuclein in C6 cells. The cell was stained with ACS0632 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant protein (20ng) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP60 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human alpha-synuclein protein
Lane 2.: Recombinant human beta-synuclein protein
Lane 3.: Recombinant human gamma-synuclein protein
Lane 4.: Mouse brain Tissue lysate
Lane 5.: Rat brain Tissue lysate

Flow cytometry (FACS)

Flow cytometry analysis of alpha, beta-Synuclein in LNCaP cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Flow cytometry analysis of alpha, beta-Synuclein in U87MG cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Flow cytometry analysis of alpha, beta-Synuclein in C6 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.