NKMAX

Product Information

Product Type

Monoclonal Antibody

Clone Number

4E7

UniProt No.

P0DMV8

NCBI Accession No.

NP_005336

Alternative names

Heat shock 70kDa protein 1A, Heat shock protein family A member 1A, HSP70, HSP70.1, HSP70-1/HSP70-2, HSPA1, HSPA1A, HSPA1B

Product Specification

Host

Mouse

Reacts With

Human

Concentration

1mg/ml (determined by BCA assay)

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Immunogen

Recombinant human Hsp70 (1-641aa) purified from E. coli

Isotype

IgG1 kappa

Purification

By protein-G affinity chromatography

Applications

ELISA, WB, ICC/IF, IHC

Usage

The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunocytochemistry/Immunofluorescence (ICC/IF)

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ICC/IF analysis of HSP70 in A549 cells line. The cell was stained with AHS0703 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

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The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp70 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: HeLa cell lysate

IHC Comment And Pic

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Paraffin embedded sections of human eccrine gland were incubated with anti-human Hsp70 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)