Human PSP antibody

Cat No.
AHP0705

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
3G12
UniProt No.
P78330
NCBI Accession No.
NP_004568
Alternative names
PSPH, Phosphoserine phosphatase Human, PSPase, PSP, EC 3.1.3.3, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, Phosphoserine phosphatase, L 3 phosphoserine phosphatase, O phosphoserine phosphohydrolase, Phosphoserine phosphatase deficiency, included,

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human PSP (1-225aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-A affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of PSP in A431 cells. The cell was stained with AHP0705 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PSP antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 2.: K562 cell lysate
Lane 3.: HepG2 cell lysate
Lane 4.: A549 cell lysate
Lane 5.: MCF7 cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.