Human VAMP-2 antibody

Cat No.
AVA0710

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
3E5
UniProt No.
P63027
NCBI Accession No.
NP_055047
Alternative names
vesicle-associated membrane protein 2, VAMP2, SYB2, Synaptobrevin 2, VAMP-2, Synaptobrevin-2, Vesicle-associated membrane protein 2, vesicle-associated membrane protein 2 FLJ11460, RATVAMPB, RATVAMPIR, SYB, VAMP 2, Vesicle associated membrane protein 2, Vesicle-associated membrane protein 2 (synaptobrevin 2)

Product Specification

Host
Mouse
Reacts With
Human, Mouse
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Synaptobrevin 2 (1-89aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of VAMP-2 in U87MG cells. The cell was stained with AVA0710 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Recombinant human VAMP1 protein
Lane 2.: Recombinant human VAMP2 protein
Lane 3.: Recombinant human VAMP3 protein
The tissue lysate (50ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse brain Tissue lysate
The tissue lysate (20ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VAMP-2 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Rat brain Tissue lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.