Human Hexokinase antibody

Cat No.
AHK0715

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
4D7
UniProt No.
P19367
NCBI Accession No.
NP_000179
Alternative names
HK1, Hexokinase 1, EC 2.7.1.1, Hexokinase type I, HK I, Brain form hexokinase, Hexokinase-1, BB404130, dea, Glycolytic enzyme, HEXOKIN, Hexokinase PI, Hexokinase type I, Hexokinase, tumor isozyme, Hexokinase-A, HK1 tb, Hk1-s, HK1-ta, HK1-tc, HKI, HXK1, mHk1-s

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Hexokinase1 (1-917aa) purified from E. coli
Isotype
IgG2a kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,ICC/IF
Usage
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of Hexokinase in HeLa cells. The cell was stained with AHK0715 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Hexokinase 1 recombinant protein
Lane 2.: Hexokinase 2 recombinant protein
Lane 3.: Hexokinase 3 recombinant protein
Lane 4.: Hexokinase 4 recombinant protein
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: HeLa cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: K562 cell lysate
The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hexokinase antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Mouse Brain Tissue lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.