Product Information
- Product Type
- Monoclonal Antibody
- Clone Number
- 3F10
- UniProt No.
- Q14653
- NCBI Accession No.
- NP_001562
- Alternative names
- IRF3, Interferon regulatory factor-3, IRF-3, Interferon regulatory factor 3, IRF 3
Product Specification
- Host
- Mouse
- Reacts With
- Human
- Concentration
- 1mg/ml (determined by BCA assay)
- Formulation
- Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
- Immunogen
- Recombinant human IRF3(108-166aa) purified from E. coli
- Isotype
- IgG1 kappa
- Purification
- By protein-G affinity chromatography
- Applications
- ELISA, WB, ICC/IF, FACS
- Usage
- The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
- Storage
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Data
Immunocytochemistry/Immunofluorescence (ICC/IF)
ICC/IF analysis of human HeLa cells stained with IRF3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.
Western blot analysis (WB)
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: MCF7 cell lysate
Lane 2.: K562 cell lysate
Lane 3.: Jurkat cell lysate
Lane 1.: MCF7 cell lysate
Lane 2.: K562 cell lysate
Lane 3.: Jurkat cell lysate
Flow cytometry (FACS)
Flow cytometry analysis of IRF3 in MCF7 cells. The cell was stained with AIR0718 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse
monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Flow cytometry analysis of IRF3 in Jurkat cells. The cell was stained with AIR0718 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse
monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.