Human Serpin A12 antibody

Cat No.
AVA0721

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
1C4
UniProt No.
Q8IW75
NCBI Accession No.
NM_173850
Alternative names
Serine (or cysteine) proteinase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 12, SERPINA12, serpin peptidase inhibitor clade A member 12, OL-64, Visceral adipose tissue-derived serine protease inhibitor, Serpin A12 precursor, OL64 OL 64, ENSG00000165953, Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 12 antibody

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human Vaspin (21-414aa) purified from E. coli
Isotype
IgG1 kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB
Usage
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Vaspin antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Vaspin transfected 293T cell lysate

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.