Human CD73 antibody

Cat No.
ATGA0554

Size

QTY

List Price

100ul

USD 320


50ul

USD 190

USD 0



Product Information

Product Type
Monoclonal antibody
Clone Number
AT89E9
UniProt No.
P21589
NCBI Accession No.
NP_002517
Alternative names
5' nucleotidase, ecto, 5'-nucleotidase ecto (CD73), 5' nucleotidase (CD73), CD73, eN, eNT
Additional Information
This product was produced from tissue culture supe

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human 5'-Nucleotidase/CD73 (27-252aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA,WB,FACS
Usage
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Western blot analysis (WB)

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 5'-Nucleotidase/CD73 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: HepG2 cell lysate
Lane 2.: WiDr cell lysate
Lane 3.: A431 cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of 5'-Nucleotidase/CD73 in A431 cells. The cell was stained with ATGA0554 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.