Human IL-33 antibody

Cat No.
AIL0824

Size

QTY

List Price

100ul

USD 290


50ul

USD 170

USD 0



Product Information

Product Type
Monoclonal Antibody
Clone Number
4E9
UniProt No.
O95760
NCBI Accession No.
NP_254274
Alternative names
Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, IL-33, Interleukin 33 CHROMOSOME 9 OPEN READING FRAME 26, DKFZp586H0523, DVS27 related protein, IL 1F11, IL 33, IL1F11, Interleukin 1 family member 11, INTERLEuKIN 33 NFHEV, Interleukin 33 precursor, Interleukin33, NF HEV, NFHEV, Nuclear factor from high endothelial venules, RP11 575C20.2, il33

Product Specification

Host
Mouse
Reacts With
Human
Concentration
1mg/ml (determined by BCA assay)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Immunogen
Recombinant human IL-33 (112-270aa) purified from E. coli
Isotype
IgG2b kappa
Purification
By protein-G affinity chromatography
Applications
ELISA, WB, ICC/IF, FACS
Usage
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Data

Immunflorescense (ICC/IF)

ICC/IF analysis of IL-33 in Jurkat cells. The cell was stained with AIL0824 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot analysis (WB)

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-33 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: 293T cell lysate
Lane 2.: Jurkat cell lysate

Flow cytometry (FACS)

Flow cytometry analysis of IL-33 in Jurkat cells. The cell was stained with AIL0824 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.